Quick Answer: What Are The 4 Methods Of Sterilization?

What are the five methods of sterilization?

Top 5 Methods Used for Sterilization | MicrobiologyMethod # 1.

Moist Heat Sterilization:Method # 2.

Dry Heat Sterilization:Method # 3.

Gas Sterilization:Method # 4.

Sterilization by Radiation:Method # 5.

Sterilization by Filtration:.

How many methods are there in moist heat sterilization?

Different methods are used to achieve sterilization. One of the most common is applying moist heat which includes autoclaving (pressure cooking), boiling, and Tyndallisation. Dry heat sterilization is accomplished by conduction and is used widely for instruments. Other heat methods include flaming and incineration.

What is the chemical method of sterilization?

To sterilize them without using heat, the recommended alternative is through chemical sterilization. Chemical sterilization is the process of using low temperature chemicals to kill, eliminate, and remove all germs, viruses, and bacteria. This can be in the form of gas or liquid chemicals.

How many types of sterilization are there?

Common methods of sterilization include physical methods and chemical methods. Physical methods include dry heat, steam, radiation, and plasmas. Radiation encompasses a variety of types, including gamma radiation, electron beam, X-ray, ultraviolet, microwave, and white (broad spectrum) light.

What is physical method of sterilization?

Physical methods: Heat is considered to be most reliable method of sterilization of objects that can withstand heat. Heat as Moist and Dry heat are the most common sterilizing methods used in hospitals and are indicated for most materials.

What are methods of sterilization?

Other Sterilization MethodsIonizing Radiation.Dry-Heat Sterilizers.Liquid Chemicals.Performic Acid.Filtration.Microwave.Glass Bead “Sterilizer”Vaporized Hydrogen Peroxide (VHP®)More items…

What are the 3 types of sterilization?

Three primary methods of medical sterilization occur from high temperature/pressure and chemical processes.Plasma Gas Sterilizers. … Autoclaves. … Vaporized Hydrogen Peroxide Sterilizers.

What is the best method of sterilization?

Classical sterilization techniques using saturated steam under pressure or hot air are the most reliable and should be used whenever possible. Other sterilization methods include filtration, ionizing radiation (gamma and electron-beam radiation), and gas (ethylene oxide, formaldehyde).

What is the fastest method of sterilization?

AutoclavingAutoclaving is probably the most common, quick, and safe sterilization method. Another thermal processing method is dry heat sterilization. In dry environments, bacterial spores can withstand higher temperatures for longer.

What Cannot be sterilized in an autoclave?

A medical autoclave is a device that uses steam to sterilize equipment and other objects. This means that all bacteria, viruses, fungi, and spores are inactivated. … Because damp heat is used, heat-labile products (such as some plastics) cannot be sterilized this way or they will melt.

How hot is an autoclave?

121° CTo be effective, the autoclave must reach and maintain a temperature of 121° C for at least 30 minutes by using saturated steam under at least 15 psi of pressure.

Which method of sterilization is used to sterilize liquids?

Microbial growth media and other liquids used for working with microbes must be sterilized to prevent contaminants from growing in them. The most effictive way to sterilize liquids is to heat them in an autoclave or a pressure cooker. But in some cases-especially for media with at least 10% salt-boiling is sufficient.

How does dry heat kill bacteria?

Dry heat helps kill the organisms using the destructive oxidation method. This helps destroy large contaminating bio-molecules such as proteins. The essential cell constituents are destroyed and the organism dies. The temperature is maintained for almost an hour to kill the most difficult of the resistant spores.

How does moist heat kill bacteria?

Moist Heat: Kills microorganisms by coagulating their proteins. In general, moist heat is much more effective than dry heat. Boiling: Heat to 100oC or more at sea level. Kills vegetative forms of bacterial pathogens, almost all viruses, and fungi and their spores within 10 minutes or less.

Does sterilization kill viruses?

A sterile surface/object is completely free of living microorganisms and viruses. Sterilization procedures kill all microorganisms. Methods used in sterilization procedures include heat, ethylene oxide gas, hydrogen peroxide gas, plasma, ozone, and radiation.

Why autoclaving is the best method of sterilization?

Autoclaves can inactivate fungi, bacteria, spores, viruses and other microorganisms on surgical instruments such as scalpels, forceps, scissors and other metal items. Because autoclaving sterilizes without the use of reagents and allows for the re-use of lab equipment and supplies, it is environmentally friendly.

What is difference between sterilization and disinfection?

Disinfection and sterilization are both decontamination processes. While disinfection is the process of eliminating or reducing harmful microorganisms from inanimate objects and surfaces, sterilization is the process of killing all microorganisms.

What are the side effects of sterilization?

Fact: There are no documented medical side effects of female sterilization. The few complications that do occur during or following sterilization, such as infection or abscess of the wound, can generally be kept to a minimum if appropriate techniques are used and if the procedure if performed in an appropriate setting.